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技術(shù)參數(shù) | |
| 壽命范圍 | From 150 ps to 3 μs |
| 儀器尺寸 | 15" x 13" x 8.8" (WxDxH) |
| 重量 | 25 lbs |
| 靈敏度 | 400 pM fluorescein or better |
| 激發(fā)光源 | OBB proprietary pulsed nanosecond LEDs |
| 脈沖寬度 | 1.5 ns (typical) |
| 激發(fā)光譜范圍 | 280–670 nm (LED dependent) |
| 發(fā)射光譜范圍 | 200–650 nm (optional to 900 nm) |
| 激發(fā)波長選擇 | By filters |
| 探測(cè)器 | OBB patented lifetime detector |
| 采集時(shí)間 | 20 s (sample dependent) |
| Timescale (menu selectable) | Linear， arithmetic and logarithmic |
| 采集模式 | Fluorescence Decay or Lifetime Kinetics |
| 樣品倉 | Single 1 x 1 cm cuvette (micro-cuvettes available) |
| 軟件 | EasyLife™ X |
| 壽命分析 | Complete package: 1-to-4 exp， global， non-exponential， micelle kinetics， lifetime distribution (ESM， MEM)， anisotropy， FRET calculator |
| QuickStart DVD | Included |
Nothing could be easier. OBB engineers have developed a broad range of simple， inexpensive， nanosecond pulsed light emitting diodes (LED's) to be used as excitation sources with the EasyLife™ systems. These small but robust light sources are available in a broad range of wavelengths， from UV to NIR. They have excellent pulse to pulse， and long term， stability in their intensity as well as their temporal pulse profiles as is evidenced by the high quality of data the system produces. Switching from one LED to another requires no alignment， and takes only a few seconds. You could not possibly work with any simpler， maintenance-free， light source for fluorescence lifetime research.
The EasyLife™ pulsed LEDs have certain distinct advantages over more conventional light sources， such as nanosecond flash lamps and lasers.
These advantages are:
Inexpensive
Durable
Require no maintenance
Very stable pulse sources
Fast
Wavelengths from UV to NIR (currently 280–670 nm)
Small size
Easily interchangeable (snap in—snap out)
6 Hours Repeat Acquisition | Repeat After 4 Days |
Click on images to enlarge. | |
What you need to do is to select a source or sources. The following list shows you the current choices. To exchange sources is real easy， just snap off and then snap on and it is done—no alignment required.
Below 280 nm we are limited by the current state of the technology for LED’s. Do not despair the future should offer even more choices.
LED Sources: | Central Wavelength |
EL 265 | excitation LED: 266 +\- 10 nm |
EL 278 | excitation LED: 280 +\- 10 nm |
EL 295 | excitation LED: 297 +\- 10 nm |
EL 310 | excitation LED: 310 +\- 10 nm |
EL 340 | excitation LED: 340 +\- 10 nm |
EL 366 | excitation LED: 368 +\- 10 nm |
EL 375 | excitation LED: 375 +\- 10 nm |
EL 405 | excitation LED: 403 +\- 10 nm |
EL 410 | excitation LED: 407 +\- 10 nm |
EL 435 | excitation LED: 432 +\- 10 nm |
EL 445 | excitation LED: 444 +\- 10 nm |
EL 450 | excitation LED: 456 +\- 10 nm |
EL 490 | excitation LED: 486 +\- 10 nm |
EL 505 | excitation LED: 510 +\- 10 nm |
EL 525 | excitation LED: 518 +\- 10 nm |
EL 570 | excitation LED: 572 +\- 10 nm |
LD 630 | excitation laser diode: 633 +\- 10 nm |
LD 650 | excitation laser diode: 649 +\- 10 nm |
LD 670 | excitation laser diode: 667 +\- 10 nm |
Other LED's and laser diodes are available on request. | |
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