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儀器簡介：快速動(dòng)力學(xué)停流裝置與光譜儀器配合進(jìn)行快速動(dòng)力學(xué)瞬間實(shí)時(shí)記錄，或離線分析，為廣大的快速動(dòng)力學(xué)研究學(xué)者提供了強(qiáng)有力的武器。選擇不同的配置可進(jìn)行下列應(yīng)用：

* 蛋白質(zhì)折疊動(dòng)力學(xué)（Protein Folding)

* 酶動(dòng)力學(xué)（Enzyme Kinetice)

* 底物結(jié)合（Substrate Binding)

* 構(gòu)象轉(zhuǎn)變（Conformational Changes)

* 胞內(nèi)底物運(yùn)輸（Substrate Transport in Vesicles)

* 第二信使研究（Second Messenger Studies)

* 快速化學(xué)反應(yīng)動(dòng)力學(xué)（Rapid Chemical Reaction Kinetics)

KinTek has always been aleader in building stopped-flow instrumentsthat conserve precious samples. The all new AutoSF-120 sets a new standard for sample economy， requiringonly 120 microliters of sample to flush， load and collect data， while giving with 3-4 replicate measurements.The flushing and sample loading areall done automatically. Simply pipette 120 microliters of solution into each sample cup and click "Load".

In the process of designing this new state-of-the-art instrument， we also added new noise reduction electronic circuits to improve signal:noise twofold and achieved asubmillisecond dead time. In addition we completely re-wrote the system software for data collection and analysis to give unparalleled ease of use and power.

The KinTek AutoSF is by far the best stopped-flow instrument ever built， giving you more high quality data while consumingless sample and less time.

An optionalrobotic autosamplerincreases throughput even more to afford unattended stopped-flow measurements ofhundreds of samples per day.

技術(shù)參數(shù)：

1.注射器數(shù)量：3個(gè)

2.驅(qū)動(dòng)方式：每一個(gè)注射器由一個(gè)步進(jìn)馬達(dá)驅(qū)動(dòng)

3.*小死時(shí)間：0.6毫秒（FC-08池）0.25毫秒（微量池）

4.注射流量范圍：0.01-10ml/s(取決于安裝的注射器）

5.混合比例范圍：1/1至1/100連續(xù)可調(diào)

主要特點(diǎn)：

1.恒溫水夾套設(shè)計(jì)，全電腦控制操作

2.程控驅(qū)動(dòng)注射器，每路樣品流量可調(diào)，不受濃度與粘度影響

3.高精度，完全可調(diào)的混合與稀釋比例

4.極短的死時(shí)間，*短為0.25毫秒

5.二路、三路、四路流動(dòng)注射停流裝置可選擇

• Small sample volume:120 μL is sufficientto flush and load a new sample and get3-4 reproducible shots.All other instruments require 3-5 times as much sample.

• Automated computer-controlledload/flush/fire cycle — simply pipet 120 μL into each sample cup and click "load".

• Twofold improvement in signal/noise with new noise reduction circuits

• Compact footprint: instrument and electronics combined (14 × 26.5 × 4.5 inches)

• Computer optimized optical path with lower noise and background fluorescence

• Automated sample loader， which can be interfaced with an optional robot.

• 200 μl of sample is sufficient to load and collect 6-8 good shots.

• Standard 10 μl flow cell.

• 20 μl per shot per solution.

• Hastwo fluorescence channelsandone absorbance channelthat can be observed and recorded simultaneously.

• Under complete computer control， courtesy of the KinTek StopFlow Software.